Human Babesia microti Incidence and Ixodes scapularis Distribution, Rhode Island, 1998–2004

Distribution of nymphal Ixodes scapularis in Rhode Island was used as a logistical regressor for predicting presence of human babesiosis. Although the incidence of babesiosis is increasing in southern Rhode Island, large areas of the state are free of babesiosis risk

Signatures of exosolar planets in dust debris disks

We apply our recently elaborated, powerful numerical approach to the high-resolution modeling of the structure and emission of circumstellar dust disks, incorporating all relevant physical processes. Specifically, we examine the resonant structure of a dusty disk induced by the presence of one planet. It is shown that the planet, via resonances and gravitational scattering, produces (1) an asymmetric resonant dust belt with one or more clumps intermittent with one or a few off-center cavities; and (2) a central cavity void of dust. These features can serve as indicators of a planet embedded in the circumstellar dust disk and, moreover, can be used to determine its major orbital parameters and even the mass of the planet. The results of our study reveal a remarkable similarity with various types of highly asymmetric circumstellar disks observed with the James Clerk Maxwell Telescope around Epsilon Eridani and Vega. The proposed interpretation of the clumps in those disks as being resonant patterns is testable -- it predicts the asymmetric design around the star to revolve, viz., by 1.2--1.6 deg/yr about Vega and 0.6--0.8 deg/yr about Epsilon Eri.Comment: to be published in ApJ Letters (v. 537, July 10, 2000), 5 pages, incl. 2 figures. Position of (color) Fig. 2 corrected to make the Figure caption fully readabl

Exploring educators’ understanding of developing learners’ reading skills and their readiness to implement CAPS

This study explored what three Intermediate Phase English First Additional Language teachers understood about reading and teaching reading, and the strategies they used to develop learners’ reading skills. Data gathered through interviews and observations of classroom practice were used to consider the extent of their readiness to achieve the aims implicit in the Curriculum and Assessment Policy Statement (CAPS). It was found that these educators understand reading primarily as the oral performance of decoding text to speech, and view comprehension as a separate and restricted entity; also, limitations in their understanding of comprehension processes involved in reading impeded their readiness to implement CAPS. Shortcomings in educators’ schooling experiences, training and professional development were considered with a view to informing future teacher training and workshops. This study formed part of a university led research project which investigated well-documented indications of poor development of literacy skills in South African schools.Keywords: Curriculum and Assessment Policy Statement (CAPS), Decoding, Interactive models of reading, Intermediate Phase (IP), Reading skills, Annual National Assessment (ANA)

Beyond arousal and valence: the importance of the biological versus social relevance of emotional stimuli

The present study addressed the hypothesis that emotional stimuli relevant to survival or reproduction (biologically emotional stimuli) automatically affect cognitive processing (e.g., attention, memory), while those relevant to social life (socially emotional stimuli) require elaborative processing to modulate attention and memory. Results of our behavioral studies showed that (1) biologically emotional images hold attention more strongly than do socially emotional images, (2) memory for biologically emotional images was enhanced even with limited cognitive resources, but (3) memory for socially emotional images was enhanced only when people had sufficient cognitive resources at encoding. Neither images’ subjective arousal nor their valence modulated these patterns. A subsequent functional magnetic resonance imaging study revealed that biologically emotional images induced stronger activity in the visual cortex and greater functional connectivity between the amygdala and visual cortex than did socially emotional images. These results suggest that the interconnection between the amygdala and visual cortex supports enhanced attention allocation to biological stimuli. In contrast, socially emotional images evoked greater activity in the medial prefrontal cortex (MPFC) and yielded stronger functional connectivity between the amygdala and MPFC than did biological images. Thus, it appears that emotional processing of social stimuli involves elaborative processing requiring frontal lobe activity

Autonomous Underwater Vehicles as Tools for Deep-Submergence Archaeology

Marine archaeology beyond the capabilities of scuba divers is a technologically enabled field. The tool suite includes ship-based systems such as towed side-scan sonars and remotely operated vehicles, and more recently free-swimming autonomous underwater vehicles (AUVs). Each of these platforms has various imaging and mapping capabilities appropriate for specific scales and tasks. Broadly speaking, AUVs are becoming effective tools for locating, identifying, and surveying archaeological sites. This paper discusses the role of AUVs in this suite of tools, outlines some specific design criteria necessary to maximize their utility in the field, and presents directions for future developments. Results are presented for a recent joint AUV–towed system survey and a demonstration of current mine-hunting technologies applied to archaeology

New measurements of the cosmic infrared background fluctuations in deep Spitzer/IRAC survey data and their cosmological implications

We extend previous measurements of cosmic infrared background (CIB) fluctuations to ~ 1 deg using new data from the Spitzer Extended Deep Survey. Two fields, with depths of ~12 hr/pixel over 3 epochs, are analyzed at 3.6 and 4.5 mic. Maps of the fields were assembled using a self-calibration method uniquely suitable for probing faint diffuse backgrounds. Resolved sources were removed from the maps to a magnitude limit of AB mag ~ 25, as indicated by the level of the remaining shot noise. The maps were then Fourier-transformed and their power spectra were evaluated. Instrumental noise was estimated from the time-differenced data, and subtracting this isolates the spatial fluctuations of the actual sky. The power spectra of the source-subtracted fields remain identical (within the observational uncertainties) for the three epochs indicating that zodiacal light contributes negligibly to the fluctuations. Comparing to 8 mic power spectra shows that Galactic cirrus cannot account for the fluctuations. The signal appears isotropically distributed on the sky as required for an extragalactic origin. The CIB fluctuations continue to diverge to > 10 times those of known galaxy populations on angular scales out to < 1 deg. The low shot noise levels remaining in the diffuse maps indicate that the large scale fluctuations arise from the spatial clustering of faint sources well below the confusion noise. The spatial spectrum of these fluctuations is in reasonable agreement with an origin in populations clustered according to the standard cosmological model (LCDM) at epochs coinciding with the first stars era.Comment: ApJ, to be publishe

Functional genomics tool: Gene silencing in Ixodes scapularis eggs and nymphs by electroporated dsRNA

Background: Ticks are blood-sucking arthropods responsible for transmitting a wide variety of disease-causing agents, and constitute important public health threats globally. Ixodes scapularis is the primary vector of the Lyme disease agent in the eastern and central U.S. RNAi is a mechanism by which gene-specific double-stranded RNA (dsRNA) triggers degradation of homologous mRNA transcripts. Here, we describe an optimized protocol for effectively suppressing gene expression in the egg and nymphal stages of I. scapularis by electroporation. Results: The genes encoding the putative Phospholipase A2 (PLA2), cytoplasmic Cystatin, Syntaxin-5, β-Actin and Calreticulin were targeted by delivering the dsRNA encoding the specific gene coding regions in the unfed nymphs. Silencing was measured using real time qRT-PCR. Electroporation as a mode of dsRNA delivery appears to be substantially efficient and less traumatic to the tick than dsRNA microinjection in the unfed nymphs. Using Cy3-labeled dsRNA to monitor the movement, electroporated dsRNA entered the nymphs and spread to salivary glands and other tissues. The significant disruption of β-actin and cytoplasmic Cystatin transcripts in tick eggs demonstrate the applicability of this technique. The PLA2, cytoplasmic Cystatin, Syntaxin-5, β-Actin and Calreticulin genes were also significantly silenced, suggesting that this method has the potential to introduce dsRNA in eggs and unfed nymphs. Conclusions: Our study demonstrates that electroporation can be used as a simple dsRNA delivery tool in assessing the functional role of tick genes in the vector-host interactions. This technique represents a novel approach for specific gene suppression in immature stages of ticks